Gel Electrophoresis Reagents and Buffers
Teknova 1M Sodium Phosphate Buffer, pH 6.0. 1000mL, Sterile.
Sodium phosphate is a versatile chemical compound that has a wide range of uses. In molecular biology, it can be used to extract DNA and RNA from cells. In protein chemistry, it can be used to purify proteins. And in biochemical applications, it can be used to study enzyme activity. Sodium phosphate is also an effective buffering agent, making it an ideal solution for use in laboratory and industrial settings. The buffer range is 5.8 to 8.0.
ABclonal Technology SSB Rabbit pAb
The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5 and 3 ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.
ABclonal Technology MCL1 Rabbit pAb
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
ABclonal Technology PPP1R12A Rabbit pAb
Myosin phosphatase target subunit 1 (MBS), also known as the myosin-binding subunit of myosin phosphatase, is a key subunit of myosin phosphatase. It regulates actin-myosin interactions downstream of the Rho GTPase. Rho is involved in myosin light chain (MLC) phosphorylation, which triggers smooth muscle contraction and actin-myosin interaction in nonmuscle cells. The active form of RhoA (GTP.RhoA) specifically interacts with MBS, regulating MLC phosphorylation. Rho-kinase, activated by GTP.RhoA, phosphorylates MBS and inactivates myosin phosphatase. Overexpression of RhoA in NIH 3T3 cells increases MBS and MLC phosphorylation. Thus, Rho inhibits myosin phosphatase via Rho-kinase. Several transcript variants encoding different isoforms have been identified for this gene.
ABclonal Technology MBTPS1 Rabbit pAb
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the cis/medial-Golgi where a second autocatalytic event takes place and the catalytic activity is acquired. It encodes a type 1 membrane bound protease which is ubiquitously expressed and regulates cholesterol or lipid homeostasis via cleavage of substrates at non-basic residues. Mutations in this gene may be associated with lysosomal dysfunction.
ABclonal Technology ACTR10 Rabbit pAb
Predicted to be involved in retrograde axonal transport of mitochondrion. Predicted to be located in cytosol, extracellular region, and secretory granule. Predicted to be part of dynactin complex.
ABclonal Technology ACLY Rabbit pAb
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
ABclonal Technology IQGAP2 Rabbit pAb
This gene encodes a member of the IQGAP family. The encoded protein contains three IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. This protein interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. It also acts as a tumor suppressor and has been found to play a role in regulating innate antiviral responses. Alternative splicing results in multiple transcript variants.
ABclonal Technology CTRB1 Rabbit pAb
This gene encodes a serine protease that is a precursor of pancreatic proteolytic enzymes. The preproprotein is synthesized in the pancreas and secreted into the small intestine, where it is activated to form the functional enzyme. The CTRB1 gene is located head-to-head with the related CTRB2 gene. Some human populations have a haplotype with a 16.6 Kb inversion region, swapping portions of intron 1, exon 1, and upstream sequences between CTRB1 and CTRB2. This inversion is linked to differential gene expression and an increased risk of chronic pancreatitis. The GRCh38 assembly represents the minor allele for SNP rs8048956 in CTRB1. SNP rs8048956 in intron 1 of CTRB2 is diagnostic for the inversion. The CTRB1 gene encodes distinct isoforms, which may undergo similar processing to generate the mature protein.
ABclonal Technology Phospho-Tau-Ser198/Ser199/Ser202 Rabbit pAb
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimers disease, Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
LICOR Odyssey, Bis-Tris, 10x8, 4-20%, 15 wells (pack of 10)
Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.
Ubpbio H-XStableProteinLadder6X500UL
The H-XStable Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The H-XStable Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
ABclonal Technology RNF114 Rabbit pAb
Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in protein polyubiquitination and ubiquitin-dependent protein catabolic process. Located in cytosol and plasma membrane. Biomarker of male infertility.